Los componentes básicos naturales que sirven como moléculas portadoras de información primaria dentro de una célula son los ácidos nucleicos. El almacenamiento y la expresión de la información genética se lleva a cabo mediante dos tipos principales de ácidos nucleicos: el ADN y el ARN.
El procedimiento mediante el cual el ADN o el ARN se separan de las proteínas, las membranas y otros componentes biológicos se conoce como aislamiento de ácidos nucleicos. La calidad de la fase de aislamiento en el proceso es crucial porque los ácidos nucleicos son esenciales para muchas de las técnicas de prueba molecular que se utilizan hoy en día. Es esencial garantizar que el rendimiento y la pureza del aislamiento de ácidos nucleicos, que es la etapa inicial de todo el proceso molecular, cumplan con las expectativas. Las aplicaciones posteriores y los dominios de investigación pueden tener diferentes expectativas. La lisis, la unión, el lavado y la elución son los cuatro procesos fundamentales de un procedimiento de aislamiento de ácidos nucleicos. Primero se altera la estructura celular para producir un lisado. Para liberar el ácido nucleico, se abren la célula y el núcleo. Los ácidos nucleicos se unen a un portador. Debido a que el ácido nucleico se ha liberado del núcleo después de la primera fase de lisado pero aún está mezclado con restos celulares y otro material insoluble, es posible separar el ácido nucleico después del paso de unión. En tercer lugar, el ácido nucleico objetivo se purifica para eliminar las impurezas restantes lavando la mezcla con ciertos tampones. Para utilizar el ácido nucleico puro, a menudo se vuelve a disolver en este paso en un tampón de elución.
| ATRIBUTO | DETALLES |
| Periodo de estudio | 2020-2029 |
| Año base | 2021 |
| Año estimado | 2022 |
| Año pronosticado | 2022-2029 |
| Periodo histórico | 2018-2020 |
| Unidad | Valor (miles de millones de USD) |
| Segmentación | Por producto, por método, por tipo, por aplicación, por usuario final |
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| Por método |
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| Por tipo | |
| Por aplicación |
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El aumento de las actividades de investigación y desarrollo en el campo de la genómica está impulsando el crecimiento del mercado de aislamiento y purificación de ácidos nucleicos. Las crecientes necesidades del sector sanitario son satisfechas por las empresas farmacéuticas y biotecnológicas que invierten fuertemente en dichas actividades. Uno de los factores que influyen en el mercado es el aumento de las asociaciones entre empresas en el sector biotecnológico.
Otro factor importante que impulsa la generación de ingresos en el mercado del aislamiento y la purificación de ácidos nucleicos es el avance de la medicina personalizada. Las biopsias de tumores cancerosos utilizan cada vez más la secuenciación de nueva generación (NGS) . Debido a que el procedimiento utiliza una prueba sencilla y común que puede identificar el cáncer en una etapa temprana, las biopsias líquidas son una opción deseable para el diagnóstico del cáncer. El mayor desafío en el tratamiento del cáncer es su diagnóstico temprano, y la NGS puede abordar con éxito este problema, lo que aumenta la demanda de tecnología NGS. Por lo tanto, se proyecta que el mayor uso de la tecnología NGS tendrá un impacto directo en la adopción de métodos de aislamiento y purificación de ácidos nucleicos.
El alto costo de los instrumentos analíticos obstaculiza el crecimiento del mercado de aislamiento y purificación de ácidos nucleicos. El costo del aislamiento y purificación de ácidos nucleicos mediante kits e instrumentos automatizados es más alto que el de los métodos convencionales. Aunque estas técnicas ofrecen beneficios significativos como simplificaciones en los procesos de aislamiento, reducciones en los tiempos de trabajo, mayor seguridad y mayor producción, el costo limita su adopción. Junto con el costo de los instrumentos se suman los gastos adicionales de reactivos, material desechable, mantenimiento y capacitación. Por lo tanto, los institutos o empresas con presupuestos más pequeños no pueden permitirse estas técnicas.
Se espera que los mercados de los países en desarrollo, como India, China, México, África y Brasil, ofrezcan oportunidades de crecimiento para el mercado de aislamiento y purificación de ácidos nucleicos. El aumento de la población de pacientes en estos países se debe principalmente al crecimiento del mercado. La industria de la salud está creciendo en estos países, lo que está dando como resultado la introducción de equipos de análisis sofisticados. Se espera que la adopción del análisis genómico aumente como resultado de estos factores.
La pandemia de covid-19 fue un catalizador positivo en el crecimiento del mercado de aislamiento y purificación de ácidos nucleicos. Se estaban desarrollando ensayos y kits específicos para la detección rápida del SARS-CoV-2. Se lanzó el kit Viral RNA Xpress para el aislamiento rápido y eficiente del ARN viral.
The Nucleic Acid Isolation and Purification market size was 4.45 billion in the year 2021 and is expected to grow at a CAGR of 36.7 %.
North America held more than 36.7 % of the Nucleic Acid Isolation and Purification market revenue share in 2021 and will witness expansion in the forecast period.
The rising research and development activities in the genomic field is driving the growth of the Nucleic Acid Isolation and purification market
The Nucleic Acid Isolation and Purification market key players include Agilent Technologies, Bio-Rad Laboratories Inc., Danaher Corporation, F. Hoffmann-La Roche Ltd, GE Healthcare, Promega Corporation, Qiagen NV, and Thermo Fisher Scientific Inc, Takara Bio, Inc., Biogenuix.
The kits and reagents segment dominated the market for nucleic acid isolation and purification. The expansion of the market is primarily responsible for the widespread use of kits and reagents for DNA and RNA isolation in the preparation of samples and libraries. The segment is also being driven by the increasing use of kits and reagents in research investigations aimed at understanding both common and uncommon genome-based disorders. The instrument segment is expected to witness fastest growth in the forecast period.
The nucleic acid isolation and purification market was dominated by the North American region. The region held the largest revenue share of 36.7 % in the year 2021. The main factor driving market expansion in the area is the presence of established companies and their work on creating technologically advanced rapid purification and isolation systems. Additionally, the increasing financing initiatives for biotechnology research are accelerating the market's growth in the area.
The Nucleic Acid Isolation and Purification market is segmented based on Product, method, type, application, end user and region.
Based on the product, market is segmented into Kit, Reagent and Instrument. The kits and reagents segment dominated the market for nucleic acid isolation and purification. The expansion of the market is primarily responsible for the widespread use of kits and reagents for DNA and RNA isolation in the preparation of samples and libraries. The segment is also being driven by the increasing use of kits and reagents in research investigations aimed at understanding both common and uncommon genome-based disorders. The instrument segment is expected to witness fastest growth in the forecast period.
Between methods used for nucleic acid isolation and purification such as column-based, magnetic beads, reagent-based, magnetic beads segment dominated the market. It accounted for a share of 37.9 %. This method provides researchers and scientists with improves assay performance. This technique reduces the need for centrifugation, which typically causes nucleic acids to break.
Based on type market is sub segmented into genomic DNA, plasmid DNA, miRNA. Due to its expanding use in COVID-19 diagnostics and rising use of purified miRNA for the creation of the cDNA library, the RNA isolation and purification segment dominated the market for nucleic acid isolation and purification in 2020, accounting for the biggest revenue share of 53.6%. Several uses for these libraries exist, including clinical diagnostics, sequencing, and gene expression monitoring.
The applications of the nucleic acid isolation and purification market include precision medicine, diagnostics, drug discovery & development, agriculture and animal research. Among these, the diagnostics segment accounted for the largest revenue share in the year 2020. The significant market share can be attributed to the growing use of DNA and RNA isolation for disease detection in regular sample processing.
Among the end users of the market, hospitals and diagnostic centers accounted for the highest share of the market. The growth of the segment was due to the increasing demand for early and accurate diagnosis of diseases. Techniques for isolating and purifying DNA/RNA are proved to be effective diagnostic tools for even hereditary illnesses such sickle cell anemia, hemophilia A, and Tay-Sachs disease.
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The Nucleic Acid Isolation and Purification market key players include Agilent Technologies, Bio-Rad Laboratories Inc., Danaher Corporation, F. Hoffmann-La Roche Ltd, GE Healthcare, Promega Corporation, Qiagen NV, Thermo Fisher Scientific Inc, Takara Bio, Inc., Biogenuix.
Recent News May 12, 2022: Danaher joined bespoke gene therapy consortium (BGTC) for rare diseases. The BGTC will generate gene therapy resources that the research community can use to streamline gene therapy development for rare disorders, making the process more efficient and less costly. April 6, 2021: Promega Opened new Research & Development Facility Supporting Science at the Edge of Innovation. Kornberg Center incorporated energizing architectural design that bridges office and meeting spaces with advanced laboratories to foster flexibility in exploration and collaboration.The Nucleic Acid Isolation and Purification market by region includes North America, Asia-Pacific (APAC), Europe, South America, and Middle East & Africa (MEA).
The nucleic acid isolation and purification market was dominated by the North American region. The region held the largest revenue share of 36.7 % in the year 2021. The main factor driving market expansion in the area is the presence of established companies and their work on creating technologically advanced rapid purification and isolation systems. Additionally, the increasing financing initiatives for biotechnology research are accelerating the market's growth in the area. US is projected to lead the nucleic acid isolation and purification market. The novel detection techniques and molecular diagnostic represented by nucleic acid hybridization and nucleic acid sequence analysis are becoming significant in the medical industry.
Asia Pacific is the region with the greatest rate of growth. Rising economic growth, substantial life sciences research, an increasing patient population, government measures that are supportive, and the existence of numerous creative biotech companies in the area are all factors that are causing the market in the region to flourish. The rise in prevalence of chronic diseases such as cancer is propelling the demand for associated diagnostics and treatments. Hence, China is expected to emerge as highly remunerative nucleic acid isolation and purification market in the forecast period.In the European region, the increased usage in covid-19 detection encouraged the growth of the market. The European Medicine Agency in the U.K. is using nucleic acid tests for emergency use for detection of virus in patients. The increased investments by governments and private agencies are fueling the market growth in the region.
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